Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation
نویسندگان
چکیده
منابع مشابه
Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation.
To assess whether heterozygosity of the donor cell genome was a general parameter crucial for long-term survival of cloned animals, we tested the ability of embryonic stem (ES) cells with either an inbred or F(1) genetic background to generate cloned mice by nuclear transfer. Most clones derived from five F(1) ES cell lines survived to adulthood. In contrast, clones from three inbred ES cell li...
متن کاملGeneration of Embryonic Stem Cell-derived Transgenic Mice by Using Tetraploid Complementation
The objective of this study was to generate transgenic mice expressing human resistin gene by using the tetraploidembryonic stem (ES) cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR, cloned into pCR 2.1 TOPO vector and constructed in pCMV-Tag4C vector. Mammalian expression plasmid containing human resistin was transfected into D3-GL ES c...
متن کاملCompletely ES Cell-Derived Mice Produced by Tetraploid Complementation Using Inner Cell Mass (ICM) Deficient Blastocysts
Tetraploid complementation is often used to produce mice from embryonic stem cells (ESCs) by injection of diploid (2n) ESCs into tetraploid (4n) blastocysts (ESC-derived mice). This method has also been adapted to mouse cloning and the derivation of mice from induced pluripotent stem (iPS) cells. However, the underlying mechanism(s) of the tetraploid complementation remains largely unclear. Whe...
متن کاملStudy on some aspects of seed viability and vigor
Influences on viability and germination of seeds have been studied intensively for many years, particularly for the numer-ous commercially important agricultural, ornamental, and timber species. High germinating seed lots may differ substantially in field emergence when sown at the same time in the same field, and/or may differ in performance after storage in the same environment or transport t...
متن کاملStudy on some aspects of seed viability and vigor
Influences on viability and germination of seeds have been studied intensively for many years, particularly for the numer-ous commercially important agricultural, ornamental, and timber species. High germinating seed lots may differ substantially in field emergence when sown at the same time in the same field, and/or may differ in performance after storage in the same environment or transport t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2001
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.101118898